HIV Testing

A Lab Exercise in Everyday Biology

Abstract

Abstract

In this experiment students will perform an Enzyme Linked ImmunoSorbent Assay (ELISA) simulation to see how it is used in the diagnosis of HIV infection. The lab is a simulation and does not use HIV antigen or human blood samples; however, it is otherwise a truly accurate depiction of the technique. Students will process samples from two different "patients" to determine whether they nave been infected with HIV. We recommend that you combine this activity with the CIBT activity entitled :AIDS Transmission" and a discussion of "safe sex" activities. Several thought provoking questions have been included at the end of the lab. You may want to require student essays or ask the students to approach these questions using the CIBT Bioethics Activity. The CIBT lab, "The Cocktail": A Magic Potion Against HIV" is a good way to discuss treatment.

Abstract

Appropriate Levels

Appropriate Levels
Regents, Honors, or AP Biology.
The hands -on experiment is extremely easy to carry out. To increase the level of the lab, more detail on immunology, on the life cycle of the HIV virus can be concluded.

Abstract

Time Required

Time Required
The activity fits nicely into an 80 minute class period. While the color reactions are developing, students can do the "AIDS Transmission activity, "The Cocktail: A Magic Potion Against HIV" computer activity on drug design, or have a discussion about the biology of the HIV virus, ethical issues, etc.

Abstract

Materials

A suitable ELISA kit is required. West Hill Bio's HIV Testing ELISA can be previewed by clicking the Supplies button above.

Copyright for this material is held by Cornell University, the Cornell Institute for Biology Teachers (CIBT), the Center for Nanoscale Systems Institute for Physics Teachers (CIPT), and or West Hill Biological Resources, Inc., 2014. This work may be copied by the original recipient from this web site to provide copies for users working under the direct supervision of the original recipient. All other redistribution of this content without the express written permission of the copyright holder(s) is prohibited.

Price:       No Charge 

Authors:
Patricia Nolan, Scotia-Glenville High School, Scotia, NY


Jim Blankenship, Cornell University, Ithaca, NY