The Properties of Enzymes: A Study of Catalase

A CIBT Lab Exercise in Molecular Biology

Abstract

Abstract

The structure and function of enzymes is a central theme in cellular and mo

Abstract

Appropriate Levels

Regents, or adaptable to Honors or AP.

Abstract

Time Required

1 period to prep students and demonstrate preparation of potato extract. 1 period to collect data. 1 period to compile class data and analyze results.

Abstract

Materials

  • 100-ml graduated cylinder (1)
  • graph paper
  • glass stirring rod
  • forceps
  • hole punch
  • marking pens
  • small beakers (3), one of which must be 50-ml in volume and transparent
  • hydrogen peroxide (as sold, 3% solution; allow ~ 50 ml per team)
  • phosphate buffer, pH 7 (optional); allow~150 ml per team
  • stop watch or clear view of a clock with a second hand
  • Whatman 2.4 cm GF/A Glass microfibre filters (purchased from Fisher Scientific catalog #987412 BX for a box of 100) or use "holes" punched from standard xerox paper - allow at least 6 per team per class
  • blender (1)
  • large funnel (1)
  • large graduated cylinder (1)
  • knife to cut the potato
  • potatoes (assume one per group of students)
  • boiled potato
  • cheesecloth from a supply company (DON’T use grocery store cheesecloth) for Part 3 (per group or for class demo)
  • test tubes (5)
  • test tube rack
  • pH solutions (3, 5, 7, 9, 11)
  • hydrogen peroxide (30% solution)
  • metric ruler
  • graph paper
  • catalase Solution (60% soln from Part 1)
Copyright for this material is held by Cornell University, the Cornell Institute for Biology Teachers (CIBT), the Center for Nanoscale Systems Institute for Physics Teachers (CIPT), and or West Hill Biological Resources, Inc., 2014. This work may be copied by the original recipient from this web site to provide copies for users working under the direct supervision of the original recipient. All other redistribution of this content without the express written permission of the copyright holder(s) is prohibited.

Price: No Charge

Authors:

Marcia Cordts, Cornell University, Ithaca NY 14853

Nancy Ridenour, Ithaca High School, Ithaca NY 14850

Harriet Bongiorno, Union-Endicott Senior High, Endicott NY 13760

Mary Kay Hickey, Dryden High School, Dryden NY, 13053