Vertebrate Development: The Japanese Medaka

A Lab Exercise in Reproduction and Development

Abstract

Abstract

Students will observe eggs collected from a freshwater killifish, the Japanese Medaka (Oryzias latipes), from freshly fertilized eggs to hatchling stage and beyond. When done in conjunction with other laboratory activities, they will determine basic trends in vertebrate development and be able to compare these trends to invertebrate development.

Appropriate Levels

Appropriate Levels

Life Science, Biology, Honors Biology, Advanced Placement Biology.

Time Required

Time Required

Teacher Preparation: Significant
In-Class Time: Two periods for the demonstration version; up to two weeks for the extended version.

Materials

Materials

  • Medaka fish (can be obtained through Carolina Biological or Ward’s Supply)
  • aquarium and other fish supplies
  • petri dishes to hold eggs (60 x 15 mm work best)
  • glass Pasteur pipettes with small end fired polished closed
  • forceps
  • compound or stereomicroscopes
  • embryo rearing solution
Copyright for this material is held by Cornell University, the Cornell Institute for Biology Teachers (CIBT), the Center for Nanoscale Systems Institute for Physics Teachers (CIPT), and or West Hill Biological Resources, Inc., 2014. This work may be copied by the original recipient from this web site to provide copies for users working under the direct supervision of the original recipient. All other redistribution of this content without the express written permission of the copyright holder(s) is prohibited.

Price: No Charge

Authors:

Jon C. Glase, Cornell University, Ithaca, NY


Paul D. Reed, Chenango Valley High School, Binghamton, NY